Figure 6
Characterization of the E7-specific memory CD8+ T cell immune responses in mice treated with HPV-16 E7 DNA vaccine in combination with DMXAA. (A) Schematic diagram of the immunization regimen of the CRT/E7 DNA vaccine and/or DMXAA. 5-8 weeks old C57BL/6 mice were vaccinated with pcDNA3-CRT/E7 DNA vaccine via gene gun delivery at day 0, 3 and 6 and DMXAA (20 mg/kg) was administered on day 3 as depicted in Figure 6A. Sixty days after the first vaccination, splenocytes were harvested and characterized for E7-specific CD8+ T cells using intracellular IFN-γ staining followed by flow cytometry analysis. ( B) Representative data of intracellular cytokine staining followed by flow cytometry analysis showing the number of E7-specific IFNγ+ CD8+ T cells in after DNA vaccination +/- DMXAA treatment. (C) Bar graph depicting the number of E7-specific IFNγ+ CD8+ T cells per 3 × 105 splenocytes ± SEM following DNA vaccination +/- DMXAA treatment. The data shown here are from one representative experiment of two performed.