Multiplex analysis to determine the serum cytokine concentration from CRT/E7 DNA vaccinated mice either treated with DMXAA. 5-8 week-old C57BL/6 mice were vaccinated with 2 μg of pcDNA3-CRT/E7 DNA via gene gun delivery. 3 days after the vaccination, the mice were treated with either 20 mg/kg of DMXAA or buffer via i.p. injection. Mouse serum was collected 5 hours later and stored at -80°C until assay. Mouse cytokines were analyzed using Bio-Plex Pro Mouse Cytokine 23-plex Assay from Bio-Rad according to manufacturer's protocol. Each sample was assayed in duplicate. The data are expressed as means ± SD.