Effect of down-regulation of ARTN on the migration of KYSE150 cells. (A) Western blotting confirmed the knockdown of artemin expression in KYSE150 cells. (B) Bar graph of the relative expression of artemin. (C) KYSE150 cells after wounding and during healing. (D) Measurement of migration distance. (E) The filters were stained with crystal violet and inspected under a microscope. (F) Quantitative measurement of invaded cells. Scale bars in microscope is 100 μm. Data are representative of each group or expressed as mean ± SEM from three separate experiments.