Table 1 Primer sequences of expression vectors construction
From: miR-223 regulates migration and invasion by targeting Artemin in human esophageal carcinoma
Gene name | Primer name | primer sequence |
|---|---|---|
miR-223 | sense primer | 5'-TGGATCCGTGTCACTCGGGCTTTACCTG-3' |
| Â | antisense primer | 5'- CGAATTCGTAGACACAGCCCAGGGCTGT-3' |
miR-105 | sense primer | 5'-TGGATCCGTGCTTATGCCCTTTAGCTATG-3' |
| Â | antisense primer | 5'- TGAATTCCTGATGGTGCCATGCTTCCTCATATG-3' |
mir-760 | sense primer | 5'-TGGATCCGAGCGCGCGCCCTCCGACCAC-3' |
| Â | antisense primer | 5'-TGAATTCCCGTTAAGCCGGGCCGGTGAC-3' |
ARTN-3'UTR | sense primer | 5'- TGAGCTCGGGCTCGCTCCAGGGCTTTGCAGAC-3' |
| Â | antisense primer | 5'- TCTCGAGATAGGGGCCAGCTCCCATGAGTG-3' |
ARTN-3'UTR-Mut | sense primer | 5'-AAGACTCTAGCAGCCCCAGAGCCCTCAC-3' |
| Â | antisense primer | 5'-GTCCCTTCACCTGTTCGGGGATGA-3' |