Figure 1From: RNA interference of argininosuccinate synthetase restores sensitivity to recombinant arginine deiminase (rADI) in resistant cancer cellsAS DNA, mRNA, and protein expression in HeLa, MCF-7, and A375 cells. (a) DAN was extracted from cells, and AS DNA was further amplified by PCR using specific primers. (b and c) Cells were treated with 1 mU/mL of rADI or PBS (as control) for 4 days, and total RNA (b) and protein (c) were extracted. PCR and Western blot were used for evaluation of AS mRNA and protein expression, respectively.Back to article page