Effect of ASsiRNA and rADI on AS protein expression in HeLa and MCF-7 Cells. Cells were seeded in 6-well plates and transfected with ASsiRNA or NCsiRNA by Lipofectamine™ 2000, respectively. After 4-day treatments with different additives, AS protein expression was analyzed both in HeLa (a) and MCF-7 (b) cells. Lipofectamine, ASsiRNA, NCsiRNA, 1 mU/mL of rADI, or combinations of these substances were used. The result of ASsiRNA and rADI in HeLa cells was not present in Figure 2a because of no viable cells after the treatment for western blotting.