Immunohistochemical detection of tissue factor and its colocalization with macrophage marker (CD13) in I and I+G hearts. (A) Immunoreactivity of tissue factor was shown in I and I+G hearts, with more prominent in the latter group. (B) Colocalization of CD13 specific for monocytes/macrophage and tissue factor in heart tissue of I+G mice. Heart sections were stained with anti-tissue factor antibody (red in left upper panel), anti-CD-13 antibody (green in right upper panel), merge (left lower panel), and H & E staining (right lower panel). Co-localization of CD13 and tissue factor expression was seen in cardiac tissue near the heavy fibrosis region, implying region of prominent inflammation. Dashed line (in sections with H & E staining) indicated region of endocardium with cardiac fibrosis seen between thrombus (left upper) and myocardium (right lower). (C) Quantitative analysis of either tissue factor or CD13 staining positive cells in both control (C) and I+G hearts were shown in diagrams, **P < 0.001 vs control.