Cardiac mRNA analysis for inflammatory markers and protein analysis for AKT and eNOS expression in I+G mice compared with I+G plus simvastatin treated mice. (A) Total mRNAs were prepared from whole heart tissues, and the levels of ICAM-1, MCP-1, tissue factor, and TNF-alpha transcripts were determined by Quantitative-PCR analysis. Note that the levels of four transcripts, especially of tissue factor and TNF-alpha reduced significantly after simvastatin administration. GADPH expression was used as a control to monitor RNA quality and concentration; **p < 0.001. (B and C) Western blot analysis of phosphorylated AKT (pAkt), AKT, eNOS, and β-actin. Lanes from left to right indicate heart tissues taken from the untreated control (C), G-CSF only (G), G-CSF with statin administration (G+St), I+G, and I+G with simvastatin administration (I+G+St). Data represent results from three independent experiments. Scanning densitometry was used for semi-quantitative analysis in compared to the Akt or β-actin levels respectively; **p < 0.001 vs control.