HBO increases visfatin protein and mRNA expression in a time-dependent manner. A, Representative Western blot for visfatin in human CAECs treated with various duration of HBO at 2.5 ATA. B, Quantitative analysis of visfatin protein levels (n = 4 per group). *P < 0.01 vs. control. **P < 0.001 vs. control. C, Quantitative analysis of visfatin mRNA levels. The values from treated human CAECs have been normalized to matched tubulin measurement and then expressed as a ratio of normalized values to mRNA in the control cells (n = 4 per group). *P < 0.01 vs. control.