TNF-α mediates the induction of visfatin by HBO. A, HBO increased TNF-α secretion from human CAECs after HBO treatment. The secreted TNF-α was measured by ELISA method (n = 4 per group). *P < 0.01 vs. control. B, Representative Western blots for visfatin protein levels in human CAECs subjected to HBO stimulation for 6 h in the absence or presence of TNF-α, TNF-α monoclonal antibody, TNF-α receptor (TNF-α R) antibody and control IgG. (C) Quantitative analysis of visfatin protein levels (n = 4 per group). *P < 0.001 vs. HBO. +P < 0.001 vs. control. ¶P < 0.001 vs. TNF-α.