The concentration-dependently suppressive effects of aciculatin on the LPS-induced production of nitric oxide (NO) and PGE
. RAW264.7 cells (2 × 105) in 24-well plates were incubated with aciculatin (1-10 μM) for 30 min, followed by stimulation with LPS (1 μg/mL) for different periods of time in the continued presence of aciculatin. Then the supernatants were collected and assayed for (A) nitrite and (B) PGE2. C. Viability of RAW264.7 cells was determined with treatment of 1-10 μM aciculatin for 48 h in comparison with the control group using the MTT assay. The data are the mean ± S.E.M. for four replicates. * p < 0.05 and ** p < 0.01 compared to the indicated groups. The experiment was performed four times with similar results.