Effects of NDUFV2 N-terminal and C-terminal truncation on mitochondrial targeting of the protein. (a) The constructs generated to express full-length and truncated NDUFV2 proteins. Full-length NDUFV2 (A), N-terminal truncation (B, △1-18 NDUFV2; C, △1-32 NDUFV2; D, △1-50 NDUFV2) and C-terminal truncation (E, △198-249 NDUFV2; F, △183-249 NDUFV2) were fused with c-myc epitope tag, and expressed in T-REx-293 cells. The number of (+) symbols indicates that the proportion of cells exhibiting FITC fluorescence have a typical punctuated staining pattern and mitochondrial colocalization in (b). The (++++) symbol indicates all of the FITC fluorescence signals in transfected cells are fully colocalized with mitochondria. The (-) symbol indicates that there is no cell producing FITC fluorescence within the mitochondrial compartment. (b) The distribution of c-myc fusion proteins was detected by anti-c-myc-FITC antibody (green color) and mitochondria were labeled by Mito Tracker Red (red color). Only merged images are shown (colocalization of expressed protein and mitochondria is indicated by yellow signals). Photos A-F are corresponding to constructs A-F shown in (a). Scale bars = 10 μ m.