Involvement of cGMP-dependent mechanism in the effect of ADM on BRR response. A: Temporal changes in BRR response of the rat that received bilateral microinjections into NTS of aCSF, Rp-8-Br-cGMP (1 nmol), ADM (0.2 pmol) or ADM plus Rp-8-Br-cAMP. Data are presented as means ± SEM; *p < 0.05 compared with control; † p < 0.05 compared with the ADM group at 10, 30 or 60 mins. B: Representative gels (inset) and quantified data showing changes in the protein level of active form PKG-I in NTS of the rat at 30 min after receiving bilateral microinjections into NTS of aCSF, ADM (0.2 pmol), ADM plus ADM22-52 (0.2 pmol), or ADM plus NPLA (250 pmol). Quantified data are presented as means ± SEM. The mean value of sham-operated control rats is represented as Basal. * p < 0.05 compared with aCSF.