ADM-induced increase in phosphorylation of nNOS. A: Representative Western blotting gels showing temporal changes in phosphorylation at Ser847 (p-nNOSS847) and Ser1416 of nNOS (p-nNOSS1416) at 10, 30, and 60 min after intra-NTS microinjection of ADM (0.2 pmol). B: Quantified data showing temporal changes in the protein level of phospho-nNOS in NTS of the rat that received bilateral microinjections into NTS of aCSF (Control; sampled at 10 min after aCSF administration), or ADM (0.2 pmol). Data are presented as means ± SEM. * p < 0.05 compared with control.