ADM-induced PKA-dependent phosphorylation of nNOS. Representative Western blotting gels showing changes in phosphorylation at Ser1416 of nNOS (p-nNOSS1416) at 10 min after intra-NTS microinjection of aCSF (control), PKA inhibitor Rp-8-Br-cAMP (PKAi; 1 nmol), ADM (0.2 pmol), or ADM plus PKAi. B: Quantified data showing changes in the protein level of phospho-nNOS in NTS of the rat that received bilateral microinjections into NTS of aCSF, PKAi (1 nmol), ADM (0.2 pmol), or ADM plus PKAi. Data are presented as means ± SEM. *p < 0.05 compared with control; #p < 0.05 compared with the ADM group.