Figure 3

Effect of vanadate on PLCβ1-Tyr-phosphorylation and SHP-2 association. Cells were cultured in the absence or presence of vanadate (1 mM) and total cell lysates (200 μg) were immunoprecipitated with anti-PLCβ1 antibody. Immunoprecipitates were subjected to Western blotting (analysed by 10% SDS/PAGE gels) and immunorevealed with mouse-anti-P-Tyr (A) and rabbit-anti-SHP-2 (B) antibodies. The figure is representative of three different and separate experiments.