Figure 1

Tumorigenesis and angiogenesis of BGC cells in nude mice. (A) Western blotting analysis of the expression of STC-1 in BGC after stable transfection. (B) Mean volumes of the tumor in each group were calculated. Cultured BGC cells and BGC stable transfection cells (10⁶ cells) were injected subcutaneously into the flank of female nude mice. Tumor volumes were measured and calculated once every two days after we can see the tumor in the flank of nude mice. (C) Immunohistochemical staining of STC-1 in tumor tissues of nude mice. STC-1 was detected on the membrane of tumor cells (D) Immunohistochemical staining of PCNA in tumor tissues of nude mice. PCNA was detected in the nucleus of tumor cells. (E) Quantification of PCNA expression(the Integrated Optical Density (IOD) of PCNA) by image pro-plus software. All histology was carried out on multiple sections from individual mice and three independent in vivo experiments. (F) Hematoxylin and eosin stained sections of the Matrigel plugs (E, endothelial-like cells; T, tumor cells; S, surrounding tissues; V, microvessels). (G) Mean vessel area was quantified in each group. (*P < 0.05, **P < 0.01).