Figure 2
From: Stanniocalcin-1 promotes tumor angiogenesis through up-regulation of VEGF in gastric cancer cells
Effects of STC-1 and VEGF on HUVEC cell proliferation, tube formation. (A) CFSE positive cells were gated and CFSE fluorescence intensity was showed in histograms. HUVEC were seeded in 12-well plates in triplicate and incubated with different culture supernatants. After 72 h, HUVEC proliferation was detected by FACS. (B) Tube formation of HUVEC induced by different culture supernatants was photographed under a microscope at ×100 magnification. (C) Effects of VEGF on tube formation of HUVEC. Tube formation of HUVECs was photographed under a microscope at ×100 magnification. (D) Mean tube length was quantified by image pro-plus software. All histogram was carried out on multiple sections and the results are representative of three independent experiments. (E) effect of isotype antibody on cell migration. IS: isotype antibody; V:VEGF neutralizing antibody; BGC/STC+IS: BGC/STC cell supernatants added with isotype antibody; BGC/STC+V: BGC/STC cell supernatants added with VEGF neutralizing antibody.