Figure 3

Global analysis of the apoE3-(72-166) proteins in PBS (pH 7.3). The SV experiment (A) was centrifuged to 42,000 rpm (circles) at 20°C for 4 h. The speed of centrofugation for SE experiments (B) was 10,000 rpm (circles), 15,000 rpm (triangles), and 20,000 rpm (squares) at 20°C each for 18 h. The solid lines in A-B are the best fit distributions from global analysis of the three discrete species model by SEDPHAT according to eq. 4. The molar mass and sedimentation coefficients of the species were floated and fitted. The residuals of each fit are shown below the panels and have a local RMSD for each channel of 0.0054 (A) and 0.0050 (B). The discrete species distribution of apoE3-(72-166) from SV (closed circles) and SE (open circles) are shown in C. The parameters by best fit are shown in Table 3.