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Table 1 Guanidine hydrochloride denaturation of apoE-(72-166) proteins with and without DHPC

From: Structural and functional characterization of human apolipoprotein E 72-166 peptides in both aqueous and lipid environments

Buffer

Protein

a (kcal mol-1)

m (kcal mol-1 M-1)

[GdnCl]0.5(M)

PBS

apoE2-(72-166)

1.93 ± 0.14

1.37 ± 0.09

1.40 ± 0.14

 

apoE3-(72-166)

1.71 ± 0.18

1.51 ± 0.13

1.13 ± 0.15

 

apoE4-(72-166)

1.52 ± 0.20

2.45 ± 0.27

0.62 ± 0.11

PBS + 50 mM DHPC

apoE2-(72-166)

1.89 ± 0.24

0.84 ± 0.11

2.25 ± 0.41

 

apoE3-(72-166)

2.18 ± 0.23

1.13 ± 0.11

1.93 ± 0.28

 

apoE4-(72-166)

1.30 ± 0.26

0.88 ± 0.15

1.48 ± 0.39

  1. aThe denaturation data were analyzed by the two-state unfolding model (eq. 1). The Rsqr of each result was from 0.975 to 0.997.
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Journal of Biomedical Science

ISSN: 1423-0127