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Table 1 Guanidine hydrochloride denaturation of apoE-(72-166) proteins with and without DHPC

From: Structural and functional characterization of human apolipoprotein E 72-166 peptides in both aqueous and lipid environments

Buffer Protein a (kcal mol-1) m (kcal mol-1 M-1) [GdnCl]0.5(M)
PBS apoE2-(72-166) 1.93 ± 0.14 1.37 ± 0.09 1.40 ± 0.14
  apoE3-(72-166) 1.71 ± 0.18 1.51 ± 0.13 1.13 ± 0.15
  apoE4-(72-166) 1.52 ± 0.20 2.45 ± 0.27 0.62 ± 0.11
PBS + 50 mM DHPC apoE2-(72-166) 1.89 ± 0.24 0.84 ± 0.11 2.25 ± 0.41
  apoE3-(72-166) 2.18 ± 0.23 1.13 ± 0.11 1.93 ± 0.28
  apoE4-(72-166) 1.30 ± 0.26 0.88 ± 0.15 1.48 ± 0.39
  1. aThe denaturation data were analyzed by the two-state unfolding model (eq. 1). The Rsqr of each result was from 0.975 to 0.997.