Overexpression of Pri/Tal peptides disrupts the DV boundary formation of the wing discs. (A-D) Expression of pri/tal (B) or pep1 (D) by dpp-Gal4 causes L3 vein expansion and notched wing in N55e11/+ background. (A'-D') Higher magnification of the insets in (A) to (D). (E-H) In all figures, Cut expression is shown in red and the misexpression clones are identified by the presence of GFP (green) and marked by the yellow dash lines in (E'-H'). (E) Cut expression at the DV boundary is not affected in clones of pri/tal1-4FS. (F) In pep1 clones, Cut levels within or adjacent to the boundaries of the clones are strongly reduced. White arrow indicates the non-autonomous suppression of Cut expression by Pep1. (G and H) Expression of pep1 (H), but not pri/tal1-4FS(G), suppress ectopic Cut expression (indicated by yellow arrows) activated by the receptor, N.