Figure 2

Induction of cyclooxidase-2 (COX-2) and matrix metalloproteinase-1 (MMP-1) expression by trypsin in human synoviosarcoma cells. Human synoviosarcoma SW982 cells were treated with trypsin in serum-free L15 medium. COX-2, MMP-1 and GAPDH expressions were assayed by western blotting and RT-PCR. Relative COX-2 and MMP-1 levels were calculated by normalizing the band densities to that of GAPDH and setting the zero controls as 100%. (A) Cells were treated with 30 nM trypsin for different time periods. COX-2 and GAPDH proteins were assayed by western blotting. (B) After trypsin treatment for 8 hours, COX-2, MMP-1 and GAPDH mRNAs in the cells were analyzed by RT-PCR and agarose gel electrophoresis, and then quantified. (C) After trypsin treatment for 8 hours, COX-2, MMP-1 and GAPDH protein levels in the cells were analyzed and quantified.