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Figure 3 | Journal of Biomedical Science

Figure 3

From: Anti-Inflammatory mechanisms of the proteinase-activated receptor 2-inhibiting peptide in human synovial cells

Figure 3

Induction of cyclooxidase-2 (COX-2) and matrix metalloproteinase-1 (MMP-1)expression by proteinase-activated receptor-2-activating peptide (PAR2-AP) in human synoviosarcoma cells. Human synoviosarcoma SW982 cells were starved in serum-free L15 medium and treated with the PAR2-AP, IL-1β, and/or trypsin. Expressions of COX-2 and MMP-1 were analyzed by western blotting, and relative levels were calculated by normalizing the band densities to that of GAPDH and setting the zero controls as 100%. (A) Cells were treated with IL-1β at 5 ng/ml or PAR-2 AP at different concentrations for 24 hours after 12 hours of starvation. (B) Cells were pretreated with PAR2-AP at different concentrations for 30 minutes and then incubated with trypsin at 30 nM for 6 hours.

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