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Figure 3 | Journal of Biomedical Science

Figure 3

From: Specific microtubule-depolymerizing agents augment efficacy of dendritic cell-based cancer vaccines

Figure 3

Determination of immune cell subsets responsible for protective immunity induced by test DC vaccines. Preparations of anti-CD4 (GK1.5), anti-CD8 (53-6.7), and anti-NK1.1 (PK136) antibodies were administered by intraperitoneal injection on day 1 in test mice prior to vaccination on days 2, 5 and 8 after tumor challenge as described in Materials and Methods. C57BL/6 mice (n = 5) were immunized intratumorally with test DC vaccines on days 7, 10 and 13 after tumor challenge as described in Materials and Methods and inoculated with B16 melanoma cells (105 cells/50 μl/mouse) on day 0. Statistical difference was calculated by log-rank (Mantel-Cox) test for mouse survival and P values of less than 0.05 were considered significant as compared with control group (*, P < 0.05).

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