Effect of different administrative routes on DC-based vaccines pulsed with specific tumor cell lysates. C57BL/6 mice (n = 6) were immunized by intratumoral (I.T.), intranodal, (I.N.) or subcutaneous (S.C.) injection with DC-based vaccines pulsed with CMQ-treated TCLs on days 7, 10 and 13 after tumor challenge. One week post vaccination, right flanks of mice were subcutaneously inoculated with B16 melanoma cells (105 cells/50 μl/mouse). During the following 45 days post tumor challenge, tumor volume (A) and survival time (B) of mice was observed and measured as described in Materials and Methods. Analysis of statistical differences among test groups is described in Materials and Methods. P values of less than 0.05 were considered statistically significant (*, P < 0.05; **, P < 0.01; ***, P < 0.001).