Figure 2

Evidence of heterogeneity in gene expression between different primary culture samples. Primary epithelial cells were grown for 14 days in Matrigel with stromal co-cultures and compared to cultures grown without stroma. RNA was prepared from single acini. Data is shown for all ten matched primary epithelial cell cultures. A) the mean fold change in gene expression levels for FGFBP1 measured by Operon microarray (black bars) or QRT-PCR (open bars). B) the mean fold change in gene expression levels for DNMBP measured by Operon microarray. C) the mean fold change in gene expression levels for CLDN6 measured by Operon microarray. Changes in gene expression detected by Operon were from the 1.1 fold gene list (p < 0.05).* insufficient sample available for QRT-PCR