Figure 5

Confocal microscopy of a single colony of mouse embryonic stem cells. A: top view of a single slice of scanning showing most eGFP signals are not overlapping with mitochondria or nuclei. B: single slice scanning from upper-side view; J-2: single slice scanning from right-side view. The arrow in B indicates some overlapping signal of truncated Msr-eGFP and mitochondria. The arrowhead in C points to the area where the truncated Msr-eGFP signals are not overlapping with mitochondria, but mainly in cytosol. The crosspoint of the horizontal green line and vertical red line points to the area we are observing. The blue lines in B and C show the current slice position for this confocal scanning.