The effects of RSV on IL-1β, TNF-α, IL-6, and NF-κB protein expression in the renal tissues of STZ-DM rats. Samples were processed to measure IL-1β (A), TNF-α (B), or IL-6 (C) using a competitive ELISA. (D) Equal amounts of proteins were resolved on 10% SDS-PAGE and blotted with NF-κB antibody. The blots were shown at the top and the quantified ratios were shown at the bottom. Results were expressed as means ± standard error (n = 5 per group). *: P < 0.05 vs. control, †: P < 0.05 vs. STZ-DM, ‡: P < 0.05 vs. DM-R0.1, IL-1β: interleukin 1β, TNF-α: tumor necrosis factor α, IL-6: interleukin 6, NF-κB: nuclear factor kappa B, CON: non-diabetic control, STZ-DM: streptozotocin-induced diabetes, DM-R0.1: DM treated with RSV (0.1 mg/kg/day) for 7 days, DM-R1: DM treated with RSV (1 mg/kg/day) for 7 days.