Figure 1

Inhibition of basal, staurosporine- or hydrogen peroxide-induced cell death of NPCs by VPA. NPCs culture were treated with 0.2 mM or 0.5 mM VPA or PBS (Vehicle) and stimulated with 100 nM staurosporine or 100 μM H₂O₂. Cells were analyzed by FACS analysis or PI staining 8 hr after the treatment as described in materials and methods. (A) The ratios of apoptotic cells were measured by FACS analysis after PI staining. (B) Quantification of FACS data (n = 3). (C) PI cytochemical staining. VPA decreased the number of PI positive cells. (D) Quantification of PI staining data (n = 6), Results are mean ± S.E.M. **P < 0.01 and ***P < 0.001 vs. Vehicle. ## p < 0.01, ### p < 0.001 vs. control. Scale bar represents 100 μm.