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Table 4 Real Time PCR amplification of mGluR5, NMDA2B and GLAST mRNA in the Cerebral cortex of experimental rats

From: Enhanced glutamate, IP3 and cAMP activity in the cerebral cortex of Unilateral 6-hydroxydopamine induced Parkinson's rats: Effect of 5-HT, GABA and bone marrow cell supplementation

Animal Status

Log RQ

 

mGluR5

NMDA2B

GLAST

Control

0

0

0

6-OHDA

3.55 ± 0.24a

2.14 ± 0.12a

-2.03 ± 0.11a

6-OHDA +5HT

2.56 ± 0.12b, f

1.65 ± 0.22b, f

-1.71 ± 0.14b, f

6-OHDA +GABA

2.64 ± 0.22b, f

1.68 ± 0.19b, f

-1.81 ± 0.08b,f

6-OHDA +BMC

3.41 ± 0.24a

2.10 ± 0.18a

-2.00 ± 0.06a

6-OHDA +5HT + BMC

1.52 ± 0.29c, e

0.89 ± 0.15c, e

-1.11 ± 0.19c, e

6-OHDA + GABA + BMC

1.84 ± 0.19c, e

0.92 ± 0.18c, e

-1.13 ± 0.12c, e

6-OHDA +5HT + GABA+ BMC

0.81 ± 0.10d

0.41 ± 0.12d

-0.32 ± 0.12d

  1. Values are Mean ± S.E.M of 4-6 separate experiments. Each group consist 6-8 rats.
  2. a p < 0.001, b p < 0.01, c P < 0.05 when compared to Control,
  3. d p < 0.001, e p < 0.01, f P < 0.05 when compared to 6-OHDA group.
  4. C - Control, 6-OHDA - 6-OHDA infused, 6-OHDA +5-HT - 6-OHDA infused treated with Serotonin, 6-OHDA +GABA - 6-OHDA infused treated with GABA, 6-OHDA +BMC- 6-OHDA infused treated with BMC, 6-OHDA +5-HT+BMC- 6-OHDA infused treated with Serotonin and BMC, 6-OHDA + GABA +BMC- 6-OHDA infused treated with GABA and BMC, 6-OHDA +5-HT + GABA+ BMC- 6-OHDA infused treated with Serotonin, GABA and BMC. The relative ratios of mRNA levels were calculated using the ΔΔCT method normalized with β-actin CT value as the internal control and Control CT-value as the calibrator.