Myocardial apoptosis at 2 and 24 hours after reperfusion (n = 8 at each time point). Myocardial apoptosis was detected by TUNEL staining. TUNEL-positive cells (black staining) increased during prolonged reperfusion. PostC significantly reduced myocardial apoptosis at both 2 and 24 hours after reperfusion. AG490 and Wortmannin attenuated the anti-apoptotic effects of PostC. *p < 0.05 compared with I/R-2 h and I/R-24 h groups respectively; #p < 0.05 compared with I/R-2 h, PostC-2 h, PostC+AG490-2 h and PostC+wort-2 h groups respectively; &p < 0.05 compared with PostC-2 h and PostC-24 h respectively.