Expression of Notch pathway components in murine macrophages. (A) Expression levels of Notch receptors and ligands in RAW 264.7 cells relative to Notch1 expression. Transcript levels were determined by real-time PCR and normalized to GAPDH. (B) Expression levels of Notch receptors and ligands with or without LPS stimulation (1 μg/ml) for 3 hours. Levels of the control group were set as 1. (C) The γ-secretase inhibitor DAPT (10 μM) did not prevent LPS-induced Hes5 and Hey2 expression. (D, E) LPS induced the formation of NICD in both dose- and time-dependent manners. (), fold change as compared to basal level; *, P < 0.05.