DAPT inhibited Notch signaling and attenuated LPS-induced inflammatory responses. DAPT (10 μM) attenuated IL-1β (B), IL-6 (C), but not TNF-α (A) secretion in conditioned medium 6 hours after LPS (1 μg/ml) stimulation. DAPT also amplified the LPS-induced sFlt-1 (D), an anti-inflammatory factor, and attenuated VEGF (E) secretion in a relatively late phase. (F) DAPT, indeed, prevented LPS-induced NICD formation and inhibited HMGB1 secretion, but not production in macrophages 24 hours after LPS stimulation. (), fold change as compared to basal level; *, P < 0.05.