Protective effect of DAPT against endotoxemia and experimental sepsis. Pretreatment with DAPT (100 mg/kg) dissolved in corn oil attenuated a sublethal dose of LPS (5 μg/kg) induced TNF-α (A), IL-1β (B), and IL-6 (C) secretion in plasma. (D) LPS-induced sFlt-1 secretion was enhanced by DAPT treatment which resulted in decreased plasma free form VEGF levels. (E) DAPT pretreatment decreased LPS-induced HMGB1 expression in liver tissues. (), fold change as compared to basal level (F) Pretreatment with DAPT partially protected mice from CLP-induced lethality (n = 10 mice per group). *, P < 0.05.