Figure 4
From: Overexpression of Insig-1 protects β cell against glucolipotoxicity via SREBP-1c
Improvement of GSIS by Insig-1 overexpression. INS-1 and INS-1-Insig-1 cells were cultured in RPMI 1640 with different glucose concentrations for 0, 24 and 72 h, medium were then changed to KRBH containing 3 mM and 20 mM glucose and then collected for basal and glucose stimulated insulin secretion. Insulin concentration was determined by rat insulin RIA. * P < 0.05; ** P < 0.01 VS exposed to 11.2 mM glucose at the same time point; # P < 0.05 VS INS-1 cells exposed to 25.0 mM glucose at the same time point.