Table 1 Sequence information on the primers used for real-time PCR
From: Overexpression of Insig-1 protects β cell against glucolipotoxicity via SREBP-1c
Gene name | Sequences for forward and reverse primers (5'-3') | Gene Bank accession number |
|---|---|---|
SREBP-1c | GGAGCCATGGATTGCACATT | L16995 |
AGGCCAGGGAAGTCACTGTCT | ||
IRS-2 | CCACACGCCTTTCGCTAGA | XM-573948 |
GTACCCCCTTCACCAAAGTCAA | ||
UCP-2 | GCATTGGCCTCTACGACTCT | NM-019354.2 |
CTGGAAGCGGACCTTTACC | ||
PDX-1 | AAACGCCACACACAAGGAGAA | NM-022852 |
AGACCTGGCGGTTCACATG | ||
GLUT-2 | CAGCTGTCTCTGTGCTGCTTGT | NM-012879 |
GCCGTCATGCTCACATAACTCA | ||
Insulin | TCTTCTACACACCCATGTCCC | NM-019130 |
GGTGCAGCACTGATCCAC | ||
FAS | GCTGCTGCTGTGGACCTCAT | X62888 |
TTTATACTGGTCGGCGGCTC | ||
GAPDH | TGGTGGACCTCATGGCCTAC | XM-344448 |
CAGCAACTGAGGGCCTCTCT |