Figure 2

Knockdown of Rab5a decreases HeLa cell motility and invasion. (A) In Control and Rab5a-siRNAs cells, the expression of Rab5a was confirmed, and the number of the cells that invaded through the matrigel-coated membrane was determined by counting the cells on the lower side of the membrane under a light microscopy (×200). (B) In Control and Rab5a-siRNAs cells, cell motility was examined with a light microscopy (×40) at the indicated time points, and the wounding width was quantified. MTT data were shown in insets. The data shown represent the mean ± S.E. (n = 3). *P < 0.05 versus Control cells, as determined by two-tailed Student's t test.