RT-PCR screening of the Phex transcripts in Kbus bony tissues. The primer sets used are shown in Additional file 3, Table S1. Oligo-dT-primed (left panel) and Phex-specific R6-primed (right panel) first strand cDNAs were prepared from Kbus (KB) and KYF (K) bony tissues. No PCR products were obtained for exon 16-18-associated regions, even with R6-primed cDNAs used as templates. The regions covered by different forward (F)/reverse (R) primers are indicated at the bottom. For further details, see the text.