17β-estradiol inhibits PGE2-promoted cellular motility in human LoVo cells. LoVo cells were pretreated with vehicle, SP600125 (JNK1/2 inhibitor, 1 μM) or 17β-estradiol (10-8M) for 1 h prior to PGE2 (10-6M) treatment for another 48 h in hydroxyurea-containing DMEM, and subsequently observed the ability of migration in LoVo cells by migration assay. At the same time, LoVo cells were treated 17β-estradiol (10-8M) in the presence or absence of ERs inhibitor, ICI 182780 (5 × 10-7M). The responses to different treatments were observed and analysis with a light microscope. *, p < 0.05 versus control;**, p < 0.01 versus control. ##, p < 0.01 versus PGE2 treatment group (mean ± SE, n = 4).