Figure 4

BMP-2 gene expression levels and BMP-2 protein concentrations in in-vitro experiments. BMP2 gene expression levels in extracts from MC3T3-E1 cells (A) or C3H10T1/2 cells (B) cultured on EVOH-CP, EVOH-BMP, or EVOH-BMP-FB for 3 or 7 days. The empty columns indicate a 3-day culture and the solid columns a 7-day culture. (C) BMP-2 concentrations in the medium from cells cultured on EVOH-CP, EVOH-BMP or EVOH-BMP-FB for 7 days. The empty columns indicate the MC3T3-E1 cells and the solid columns the C3H10T1/2 cells. The values presented are the mean ± standard deviation. (n = 3, *p < 0.05, **p < 0.01, ***p < 0.001).