Figure 7

Evaluation of the animal experiments. A; Histological sections of the bone defects were stained with hematoxylin and eosin after demineralization. The bone defects were treated with HAP-CP, HAP-BMP or HAP-BMP-FB 8 weeks ago. The yellow dotted lines show the area of bone formation (indicated by new bone). Bone formation was observed between the cranium and the dural membrane, resulting in increased cranial thickness. Bone formation was also observed in the bone defect space as the extension of new bone. Bars indicate 100 μm. B; Bone formation was quantified in each group. The extension of new bone into the space left by the bone defect (open columns). The increased thickness of the cranium due to the bone formation (solid columns). The values presented are the mean ± standard deviation. (n = 5, *p < 0.05, **p < 0.01) C; ALP and OC gene expression in the bone defect tissue 8 weeks after the procedure. Open columns indicate ALP gene expression and solid columns indicate OC expression. The values presented are the mean ± standard deviation (n = 5, *p < 0.05, **p < 0.01).