Figure 4
From: Androgens as therapy for androgen receptor-positive castration-resistant prostate cancer
Effect of androgen on cell proliferation, cell cycle, and cell cycle-related proteins in hormone-dependent 104-S and androgen ablation-resistant 104-R1 cells. (A) LNCaP 104-S and 104-R2 cells were treated with increasing concentration of R1881 for 96 hours. Relative cell number was determined by using a 96-well proliferation assay and data were normalized to number of 104-S cells at 0.1 nM R1881. Asterisk (*) represents statistically significant difference between treatment group compared to control group of 104-S or 104-R1 cells. (B) Percentage of 104-S and 104-R1 cells in S phase determined by flow cytometry. LNCaP 104-S and 104-R2 cells were treated with increasing concentrations of R1881 for 96 hours. Values represent the mean + standard error derived from 5 independent experiments. (C) Protein expression of androgen receptor (AR), prostate specific antigen (PSA), p21cip, p27Kip, phosphor-retinoblastoma protein (Rb), c-Myc, S phase kinase-associated protein 2 (Skp2) were determined by Western blotting assay in 104-S and 104-R1 cells treated 96 hrs with different concentration of R1881. β-actin was used as loading control.