( A ), ( C ): The blots show the examples of immunobands against NR1 ( A ) and NR2B ( C ) and β-actin antibodies on the membrane, which was performed on the membrane protein prepared from the lumbar spinal cords of the offspring (P14) rats in different groups. (B), (D): The quantitative change of NR1 (B) and NR2B (D) protein expression. The values of NR1 and NR2B subunits in the control group were arbitrarily set at 100%. Data are presented as mean ± SEM. One-way ANOVA followed by the Newman-Keuls test was used to analyze the data. (B: *p < 0.05 for the morphine group vs the control group; D: **p < 0.001 for the morphine group vs the control group; *p < 0.05 for the M + DM group vs the control group; ¶p < 0.01 for the M + DM group vs the morphine group, n = 3).