Ceftriaxone reduced hypoxic-ischemic cell damage in the hippocampus. A, B, in situ cell death detection by TUNEL reaction in hippocampus of P14 rat brains counter-stained with hematoxylin. (A: ceftriaxone 200 mg/kg, B: saline, scale bar = 200 μm). C, the brains were evaluated under 200X light microscope in 3 separate fields each of CA1, CA2, and CA3 (total 9 fields) and summed for each animal. Pre-treatment with 100 mg/kg and 200 mg/kg ceftriaxone significantly reduced the TUNEL positive cell. one-way ANOVA, p = 0.0017 (n = 5 in each group); *: p < 0.05. Saline group refers to animals received hypoxic-ischemic procedures and saline administration.