Reduced cardiomyocyte size and disturbed gap junction distribution in the left myocardium of mice with long-term alcohol ingestion. Immunocofocal images of cardiac muscle double stained for WGA (A through D) and Cx43 (E through H). Images in each column are of the same field. Compared to the control group (A and B) the cardiomyocytes in the alcohol group (C and D) are smaller in size, but contain more Cx43 gap junctions, as shown in the below histograms, each of which represents more than 80 randomly selected cells from three animals of each group. A and C are taken from the epicardial side, B and D from the endocardial side of the left ventricular free wall. Note that the labels of WGA are more prominent in the alcohol group. In each histograms * and + respectively indicate p < 0.01 and p < 0.05 compared to the corresponding bar of the control group. See text for details. All images are of the same magnification. Bar, 30 μm.