Figure 6

Myosin of zebrafish embryos was stained by the heart-specific anti-myosin antibody, MF20 (green), and counterstained by DAPI (blue) for nuclear observation. A-F: The expression of MF20 in embryos with tbx5 deficiency (B, D, F) decreased throughout the developmental stages compared to that in the WT (A, C, E), from 24 to 48 hpf. The anterior of embryos is to the left. h, heart; MO, tbx5-MO-treated embryos.