Figure 5

The entry of Cpc into B16F10 melanoma cells. Cells were treated with Cpc (0.1 mg/mL). (A) Confocal microscopy of Cpc localization at 6 hrs after treatment (1000 ×). (B) After washes with PBS, cells were lysed, and the extract proteins were analyzed by immunoblotting assay for Cpc at the assigned time intervals (β-subunit (black); α-subunit (grey)).