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Figure 5 | Journal of Biomedical Science

Figure 5

From: BACE-1 inhibition prevents the γ-secretase inhibitor evoked Aβ rise in human neuroblastoma SH-SY5Y cells

Figure 5

Treatment with high concentration of γ-secretase inhibitor LY450139 followed by low concentration of LY450139 in SH-SY5Y cells. Treatment with 3 nM LY450139 after inhibition by 300 nM LY450139 leads to increased Aβ40 secretion in SH-SY5Y APPwt cells but not in APPswe cells. SH-SY5Y APPwt cells (A) or APPswe cells (B) were treated with vehicle (0.1% DMSO) or 300 nM γ-secretase inhibitor LY450139 for 24 h, after which the medium was collected for Aβ40 measurements (black bars). The media was replaced with fresh medium (left panel and middle panel) or 3 nM LY450139 (right panel) for another 24 h, and then again collected for Aβ40 measurements (grey bars).

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