Combining BACE- and γ-secretase inhibitor in SH-SY5Y APPswe cells. A. SH-SY5Y APPswe cells were treated with the BACE-inhibitor alone or in combination with 3 or 30 nM γ-secretase inhibitor LY450139 for 24 h. Aβ40 levels were subsequently measured in the medium with ELISA. The BACE inhibitor produced a concentration-dependent inhibition of Aβ40-secretion with it's potency shifted to the right in the presence of 3 nM LY450139. At 30 nM, LY450139, together with the BACE inhibitor abolished Aβ40-secretion. B. The same data as in Figure 7A but depicted in a different manner. Here one can see how various concentrations of the BACE inhibitor (0: open bars, 3 nM: grey bars, 30 nM: black bars) affect the response to 0, 3 and 30 nM LY450139. The figure highlights that 3 nM LY450139 seems to oppose the inhibition evoked by the BACE inhibitor.