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Figure 1 | Journal of Biomedical Science

Figure 1

From: Involvement of lipid rafts in adhesion-induced activation of Met and EGFR

Figure 1

Ligand-independent activation of Met and EGFR confers proliferative and invasive advantages to A431 cells. (a) An equal amount of whole cell lysates from epidermal carcinoma A431 cells, lung adenocarcinoma A549 cells, and bladder carcinoma T24 cells was analyzed by immunoblotting with antibodies as indicated. The activation of Met and EGFR were detected by anti-Met pY1234/1235 (p-Met) and anti-EGFR pY1173 (p-EGFR). (b) An equal amount of whole cell lysates from the A431 cells stably expressing shRNAs specific to Met (sh-Met clone #1 and #2), EGFR (sh-EGFR clone #1 and #2) or luciferase (sh-Luc) was analyzed by immunoblotting with anti-Met and anti-EGFR. β-tubulin was used as an internal control. (c) The cells as described in the panel (b) were seeded on 6-cm dishes and the cell number was counted after 4 days. Data are quantified and expressed as percentage relative to the level of the control A431 cells, which is defined as 100%. Values (means ± s.d.) are from three independent experiments. *p < 0.05 (compared with the sh-Luc). (d) The cells as described in the panel (b) were subjected to a Matrigel invasion assay. Data are quantified and expressed as percentage relative to the level of the control A431 cells, which is defined as 100%. Values (means ± s.d.) are from three independent experiments. *p < 0.05 (compared with the sh-Luc)

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